Clontech two-hybrid manual

Practice of the two-hybrid system is covered by U.S. Patents #5,, and #5,, assigned to the Research Foundation of the State University of New York. Purchase of any BD Biosciences Clontech two-hybrid reagent does not imply or convey a license to practice the two-hybrid system covered by these patents. Commercial. the two sample groups were not statisti-cally significant (P ). Thus, sub-stitution of dUTP (at 3× concentration) for dTTP did not reduce amplification efficiency for this sequence. Similarly, the use of dUTP did not significantly alter amplification efficiency of a bp segment of U2, another multicopy gene (3) (data not shown). The. Practice of the two-hybrid system is covered by U.S. Patent Nos. 5,,, 5,,, and 5,, assigned to the Research Foundation of the State University of NewYork. Purchase of any Clontech two-hybrid reagent does not imply or convey a license to practice the two-hybrid system covered by these patents.

Practice of the two-hybrid system is covered by U.S. Patent Nos. 5,,, 5,,, and 5,, assigned to the Research Foundation of the State University of NewYork. Purchase of any Clontech two-hybrid reagent does not imply or convey a license to practice the two-hybrid system covered by these patents. Matchmaker Gold Yeast Two-Hybrid System User Manual () www.doorway.ru Takara Bio USA, Inc. Page 4 of 41 I. Introduction Protocol Overview Principle of the Two-Hybrid Assay In a Matchmaker GAL4-based two-hybrid assay, a bait protein is expressed as a fusion to the Gal4 DNA -binding domain (DNA-BD), while libraries of prey. practice the two-hybrid system covered by these patents. Commercial entities purchasing these reagents must obtain a license from the Research Foundation of the State University of New York before using them. CLONTECH is required by its licensing agreement to submit a report of all purchasers of two-hybrid reagents to SUNY Stony Brook.

2. Yeast two-hybrid (Y2H) experiment was used to identify the binding System 2 kit according to the manufacturer's manual (Clontech. Plasmid pGBkT7-ECRG2 was used as a bait in two-hybrid screening of human fetal liver cDNA libraries by MATCHMAKER Two-Hybrid System 3 protocol (Clontech). The. The Y2H system that was used is. MatchmakerTM Gold Yeast Two-Hybrid System (Clontech,. USA). According to the manual, mating efficiency that will.